Journal: Molecular microbiology

Article Title: Loss of Hfq activates the sigmaE-dependent envelope stress response in Salmonella enterica.

doi: 10.1111/j.1365-2958.2006.05413.x

Figure Lengend Snippet: Fig. 4. Effect of hfq and rseA mutations on the levels of OmpC, OmpF and OmpA in the outer membrane of Salmonella enterica serovar Typhimurium. Cultures were grown in rich medium (LB; 5 g NaCl per litre) to an OD600 of 0.35. Outer membrane proteins were prepared as in Santiviago et al. (2003) and fractionated by SDS-PAGE on a 13% acrylamide gel. Only the portion of the gel with the bands corresponding to the major porins is shown. Lane 1, strain MA8740 (ompD::Tn10); lane 2, strain MA8741 (Dhfq ompD::Tn10); lane 3, strain MA8783 (DrseA ompD::Tn10); lane 4, strain MA8795 (DrseA DmicA ompD::Tn10). The complete genotypes of the strains used are in Table 2.

Article Snippet: For acrylamide gels, transfer of the RNA onto Hybond-N+ membrane (Amersham), was performed with a semidry electrotransfer apparatus (Transblot SD; Bio-Rad); in the case of agarose gels, transfer to the same support was performed using a vacuum blotter (Boekel/Appligene) after mild denaturation treatment in 50 mM NaOH, 10 mM NaCl.

Techniques: Membrane, SDS Page, Acrylamide Gel Assay